Single-cell studies are essential for understanding the complexity and heterogeneity of cell populations. Manel Juan, the head of the IDIBAPS research group Immunogenetics and immunotherapy in autoinflammatory and immune responses, recently donated the BD Rhapsody HT Single-Cell Analysis System to the Genomics Platform.
This device uses a microwell system to isolate a cell or nucleus whilst maintaining its integrity and minimising cell loss. BD Rhapsody uses a scanner to visualise cells in real time at key points in the processing.
This high-throughput system uses eight-lane cartridges where up to 12 samples can be multiplexed per lane. As such, it can analyse up to 100,000 cells per lane (800,000 cells per cartridge) and can work with highly diluted samples (starting from 200 cells/µl).
Its applications include studying the entire transcriptome (WTA) and performing targeted mRNA, Ab-Seq, TCR/BCR and ATAC-Seq.
If you are interested in using BD Rhapsody for single-cell studies, please contact the Functional Genomics Platform by email at: IDIGENOM@recerca.clinic.cat or by calling extension 2184.
